【摘要】 以兼具生态和能源植物功能的木本模式植物——杨树(河北杨)为材料,研究了携带促生长基因(35S-DAS5)的根癌农杆菌载体介导的河北杨遗传转化若干因素对转化效果的影响。结果显示,较适宜的转化系统为预培养2-4 d,农杆菌菌液(OD600值为0.4)侵染20 min,共培养4 d,在含30 mg/L卡那霉素(Km)的培养基上诱导不定芽,生根培养基中Km的适宜浓度为10 mg/L。更多还原

【Abstract】 Several factors affecting transformation of Populus hopeiensis were studied by infecting with Agrobacterium tumefaciens GV3101,such as pre-culture time,adding AS,Agrobacterium concentration,infection time,co-culture time,concentration of kanamycin used for selection and adding time,of which the frequency of kanamycin resistance buds was used as measure standard.The superior transformation system was as following,pre-cultured 2-4 days and then infected 20 minutes in Agrobacterium diluted(OD600=0.4),co-cultured 4 days,transgenic tissues and shoots were selected with 30 mg/L Km and rooting stage with 10 mg/L Km.更多还原