【摘要】 目的:采用高效液相色谱法对发酵液中的紫杉醇进行测定。方法:将紫杉醇产生菌发酵产物经乙酸乙酯萃取得测定样品,高效液相色谱分析方法为苯基柱(250mm×4.6mm,5μm),流动相乙腈-甲醇-水(36∶4∶60),流速1mL/min,检测波长227nm,进样体积20μL,柱温室温。结果:紫杉醇与干扰物可达到基线分离,在2.2～110μg/mL范围内,紫杉醇的峰面积与浓度线性关系良好,相关系数0.9996,平均回收率为99.55%,RSD为0.60%。结论:与使用C18柱色谱条件相比,该分析方法灵敏度高,不需要复杂的样品前处理过程,仪器配置不复杂、操作方便、准确性高,可有效地检测发酵液中紫杉醇的含量。更多还原

【Abstract】 Objective:The content of taxol in fermentation broth was detected by HPLC.Method:The analytic samples were obtained from ethyl acetate extract of fermentation product of taxol producing strain.The HPLC separation was performed with benzene analytical column(250mm×4.6mm,5μm)in the flow rate of 1mL/min,the mobile phase was acetonitrile-methol-water(36∶ 4∶ 60),the injection volume was 20mL,the column temperature was room temperature.Result:Taxol and interfering substances can reach baseline separation.The calibration curve of peak area and taxol concentration was linear in the concentration range of 2.2-110μg/mL with good correlation coefficient(r=0.999 6).The average recovery rate was 99.55%,RSD 0.60%.Conclusion:The convenient analytical method on benzene column with high sensitivity and high accuracy did not require complicated sample pretreatment and complex instrument configuration,could be used to determine the content of taxol in fermentation broth effectively,compared to chromatographic condition on C18 column.更多还原