【摘要】 采用试剂γ-氯丙基三乙氧基硅烷(CPTES)对介孔硅材料SBA-15进行表面改性,并通过红外图谱(FT-IR)和N2吸附脱附等温图(BET)对其进行表征。结果表明:改性前原材料的比表面积为460.9 m2/g,改性后材料比表面积提高到512.0 m2/g。利用改性前和改性后的SBA-15对猪胰脂肪酶进行固载实验,并对实验结果进行比较,发现改性后的SBA-15在脂肪酶活性、pH环境适应性、热耐受性和可操作性都优于改性前的SBA-15,在最优条件下的酶活力提高超过60%。更多还原

【Abstract】 Media for enzyme immobilization were prepared by modifying mesoporous silica SBA-15 with γ-chloropropyl triethoxysilane(CPTES) group.The prepared support was characterized with FT-IR and BET method and exhibited by high specific surface area and uniform pore size.Furthermore,unmodified SBA-15 and the modified SBA-Cl materials were used for porcine pancreas lipase immobilization.The results were compared with that of enzyme activity,activity retention and stability.The modified SBA-15 material with CPTES showed good performances for porcine pancreas lipase immobilization.Compared with unmodified SBA-15,the adsorption capacity of SBA-Cl to porcine pancreas lipase was improved more than 60%.更多还原