【摘要】 获得兔骨保护素(OPG)基因并分析序列。从兔肱骨中提取总RNA,逆转录形成cDNA,用5′RACE策略扩增OPG基因,经琼脂糖凝胶电泳鉴定,并测序及进行序列分析。兔OPG基因全长1 540bp,编码400个氨基酸,与人OPG氨基酸序列相比,同源性为89%,而与大鼠等其它动物的同源性则在85%左右。5′RACE法成功获得了兔OPG基因的真实序列,为OPG基因的功能研究奠定了良好基础。更多还原

【Abstract】 This paper is to show a way of acqusition of the v ariable region gene of rabbit osteoprotegerin(OPG) and to analyse series.Total RNA was extra cted from rabbit tibia,transcripted reversely into cDNA with random primers.Th e variable region of the OPG gene ampliflied using 5′RACE.Sequencing was confi rmed by agarose gel electrophoresis and sequencing analysis.Full length of OPG gene was 1540bp that encoding 400 amino acids.It shared 89% identity with human OPG in whole amino acid sequence and about 85% with rattus norvegicus and other mammal.The OPG sequence of rabbit was obtained by 5′RACE,which co uld provide a good basis for OPG functional study.更多还原