【摘要】 本实验探讨小鼠骨髓移植中影响死亡率的因素。将受鼠γ射线全身照射,分别经尾静脉输入同品系供鼠骨髓细胞或骨髓细胞和脾细胞的混合物。实验分4批进行,分别选用不同品系的小鼠,观察移植物抗宿主病(GVHD)表现,监测移植鼠35d生存率。结果显示第1批小鼠死亡率最低,第2批小鼠死亡率最高,且死前有明显的GVHD表现,第3批小鼠死亡率有所下降,与第2批小鼠死亡率相比,差异有统计学意义,第4批小鼠死亡率比第3批有所升高,但仍显著低于第2批小鼠,差异亦有统计学意义。由此推测小鼠基因的纯和度、脾细胞的输入、骨髓细胞和脾细胞的比例、周龄是影响小鼠骨髓移植死亡率的重要因素。更多还原

【Abstract】 The purpose of the study was to investigate the inf luencing factors of mortality rate in the bone marrow transplantation in mice.The recipient mice r eceiving whole-body irradiation of γ-ray were infused with the same strain of bone marrow cells or the mixture of the bone marrow cells and splenocytes respe ctively.Experiments were carried out in four batches,with different strains of mice used,respectively.The manifestations and the appearance of graft-versus-host disease(GVHD) were observed,as well as the mortality rate within 35 d of the transplantation in the recipient mice.The mortality rate of the first group of recipient mice was the lowest,the mortality rate of the second group o f recipient mice was the highest and the obvious GVHD performance was observed b efore death.In the third group,the mortality rate declined and there was stati stical significance compared to that of the second group.The mortality rate of the fourth group of mice was higher than that of the third group,but still lowe r than that of the second group of mice and there is a statistical significance. This evidence suggested that mouse genetic purity,splenocytes,the ratio of th e bone marrow cells and splenocytes and the week-old of the mouse could be the important influencing factors of the mortality rate in mouse bone marrow transplan tation.更多还原