【摘要】 目的:探讨汉族人群中血管紧张素转换酶抑制剂(ACEI)所致咳嗽与血管紧张素转换酶(ACE)基因及缓激肽β2受体(BDK-RB2)基因多态性的关系。方法:应用聚合酶链反应(PCR)方法,检测汉族人群中151例由于服用ACEI引起的咳嗽患者及151例未发生咳嗽的患者的ACE I/D及BDKRB2 C/T的多态性,并采用紫外法检测ACE活性。结果:发现ACE基因分布在咳嗽组中II型为47.0%,ID型为42.4%,DD型为10.6%;无咳嗽组分别为39.7%、47.0%、13.3%,两组相比其差异具有统计学意义(P<0.01);BDKRB2基因分布在咳嗽组中CC型为21.3%,CT型为50.0%,TT型为28.7%,无咳嗽组分别为22.5%、47.7%、29.8%,两组相比其差异无统计学意义(P>0.05);咳嗽组ACE活性水平为([28.3±10.1)U/L]明显低于无咳嗽组([40.2±9.4)U/L],两组相比其差异具有统计学意义(P<0.01)。结论:汉族人群中ACEI所致咳嗽与ACE基因多态性及血清ACE水平有关,BDKRB2 C/T与咳嗽间未发现有统计学意义的关联。更多还原

【Abstract】 Objective: To investigate the association between angiotensin converting enzyme inhibitor(ACEI)-induced cough and the polymorphism of angiotensin converting enzyme(ACE) gene and bradykinin β2 receptor(BDKRB2) gene in Han nationality.Methods: Polymerase chain reaction(PCR) was used to examine ACE I/D and BDKRB2 CT polymorphism in 151 ACEI-induced cough subjects and 151 no cough subjects in Han population.And UV-method was used to detect the ACE activity.Results:ACE gene distribution in the cough group: type II was 47.0%,ID genotype was 42.4%,DD genotype was 10.6%;Non-cough group were 39.7%,47.0%,13.3% respectively,and there was statistically significant difference between the two groups(P<0.01);BDKRB2 Gene distribution in the cough group: the CC genotype was 21.3%,CT genotype was 50.0%,TT genotype was 28.7%,non-cough group were 22.5%,47.7%,29.8% respectively,and there was not statistically significant between the two groups(P> 0.05);The level of ACE activity in the Cough group [(28.3 ±10.1) U / L,] was significantly lower than non-cough group [(40.2 ± 9.4) U / L,(P <0.01).].Conclusions: For han population,ACEI-induced cough related to ACE gene polymorphism and serum ACE activity,and there was no statistically significant association between BDKRB2 C / T and cough.更多还原