【摘要】 目的:评价噻唑蓝(MTT)法检测药物对细胞的毒性作用的可靠性。方法:大鼠肺泡上皮L2细胞以叔丁基对苯二酚(TBHQ)10-100μM,BSO以1-10mM分别处理,用MTT法检测细胞活性、JC-1(5,5’,6,6’-四氯-1,1’,3,3’-四乙基苯并咪唑羰花青碘化物)荧光染料法检测细胞线粒体电位改变、台盼蓝排斥实验检测细胞死亡率,分析各指标的情况。结果:在处理剂量范围,MTT法检测到的光密度(OD)值未能达到一般判断的半数抑制浓度(IC 50)水平,最高抑制率仅达到30%左右;台盼蓝排斥试验检测数据表明TBHQ的LC 50值为50μM,丁硫氨酸亚砜胺(BSO)为5 mM;利用JC-1荧光染料判断的半数凋亡剂量分别为50μM和7 mM。结论:MTT法作为最常采用的细胞生长抑制检测手段,但在某些特定实验中可能不能客观地反映细胞的活性,建议多种方法结合进行评价。更多还原

【Abstract】 Objective:To evaluate the reliability of toxic effects of drugs using MTT assay.Methods:The rat alveolar epithelial L2 cells exposure to TBHQ 10-100 μM,BSO 1-10 mM,respectively,using MTT assay to detect the activity of cells,JC-1 fluorescent dye to detect the changes in mitochondrial potential,trypan blue exclusion to detect the prevalence.Results:In the treatment dose range,the OD values detected by MTT assay failed to meet generally determine the IC 50 level,and the highest inhibition rate is around 30%;trypan blue exclusion assay data indicate that the LC 50 value of TBHQ is 50 μM,BSO to 5 mM;using of JC-1 fluorescent dye to determine the half dose is 50 μM and 7 mM,respectively.Conclusion:MTT assay is the most common means to detect the cell growth inhibition,but it may not objectively reflect the activity of cells in a given experiment.We suggest to use a variety of methods in conjunction.更多还原