【摘要】 目的:探讨经IFN-γ刺激后,人外周血单个核细胞OX40L表达的变化,以及辛伐他汀对单个核细胞OX40L表达的影响。方法:将实验标本随机分为2组,分别干扰素-γ(IFN-γ)刺激组、辛伐他汀干预组。应用RT-PCR及Western blotting技术,观察IFN-γ诱导的人外周血单个核细胞OX40L表达情况及辛伐他汀对人单个核细胞OX40L表达的影响。结果:1.1000 U/ml IFN-与人单个核细胞共同培养24h后,OX40L mRNA和蛋白水平的表达明显增加。2.预先给予10 mol/L的辛伐他汀干预1h可以明显降低IFN-诱导的OX40L表达。结论:IFN-可诱导人单个核细胞OX40L表达。辛伐他汀可以抑制IFN-诱导人单个核细胞OX40L表达的增强,从而可能抑制了OX40L信号通路介导的与炎症有关的血管损伤,延缓动脉粥样硬化的进程。更多还原

【Abstract】 Objective: To observe the effect of simvastatin on serum levels of soluble OX40L(sOX40L) in PBMCs.Methods: Human peripheral blood mononuclear cells(PBMCs) were isolated,and the effects of simvastatin on OX40L expression were evaluated by RT-PCR and western blot.Results: OX40L mRNA and protein levels increased after stimulation with 1000 U/ml IFN-where simvastatin could antagonize this increase at a concentration of 10 mol/L.Conclusions: Simvastatin treatment induced down-regulation of OX40L mRNA and protein expression in a concentration-dependent manner in human peripheral blood mononuclear cells(PBMCs).IFN-up-regulates the expression of the mRNA and protein of OX40L and this up-regulation could be reversed in present of simvastatin.更多还原