【摘要】 目的:研究脾虚模型大鼠脑内白细胞介素1β(IL-1β)、白细胞介素2(IL-2)的活性表达,以及扶正益气中药的干预作用。方法:40只大鼠随机分成4组,正常组、模型组、治疗1组(四君子汤组)、治疗2组(玉屏风散组),每组各10只。采用苦降泻下、饮食失节加劳倦过度法建立大鼠脾虚模型,采用免疫组化法检测下丘脑腹侧核、海马CA1区的IL-1β和IL-2表达变化与四君子汤玉屏风散的治疗作用。结果:IL-1β在海马CA1区和下丘脑腹侧核表达明显降低,IL-2在海马CA1区和下丘脑腹侧核表达明显降低;四君子汤治疗组IL-1β和IL-2在上述脑区表达明显上升;玉屏风散治疗组IL-1β和IL-2在上述脑区表达呈紊乱变化现象。结论:益气扶正中药四君子汤、玉屏风散可能通过影响免疫器官、调控细胞因子IL-1β和IL-2活性表达而调节机体免疫功能。更多还原

【Abstract】 Objective: To study the changes of expressions of interleukin-1β,interleukin-2 in spleen-deficiency rat model,and the interventional effects of Chinese medicinal with actions of replenishing qi and reinforce the healthy qi.Methods: All rats(n = 40) were randomly divided into the normal group,model group,treatment group 1(T group 1,treated with Sijunzi Decoction) and treatment group 2(T group 2,treated with Yupingfeng Powder,each n = 10).The spleen-deficiency model was established in rats by using the methods of purgation,improper diet and over strain.The expressions of IL-1β,IL-2 in the ventral nucleus of hypothalamus and CA1 zone of hippocampus,and therapeutic effects of Sijunzi Decoction and Yupingfeng Powder were detected by applying immunohistochemistrical assay and in situ hybridization.Results: In the model group the IL-1β,IL-2 expression decreased significantly.In T group 1 the IL-1β,IL-2 expressions increased significantly;In T group 2 changed disorderly in the ventral nucleus of hypothalamus and CA1 zone of hippocampus.Conclusion: Sijunzi Decoction and Yupingfeng Powder,with the actions of replenishing qi and reinforce the healthy qi,can regulate the immunologic function of the body through influencing immune organs and controlling the expressions of brain IL-1β and IL-2.更多还原