【摘要】 目的:通过免疫组织化学方法检测PTEN基因在正常甲状腺组织、甲状腺良性肿瘤组织、甲状腺乳头状癌癌组织中的表达水平并进行比较,探讨其对甲状腺乳头状癌诊断和治疗的意义。方法:采用SP免疫组化方法,用已知阳性组织做阳性对照,以磷酸盐缓冲液(PBS)代替一抗做阴性对照,分别作HE染色和免疫组织化学染色。结果:PTEN蛋白在三组组织中的表达差异具有显著性(P<0.001);正常甲状腺组织、甲状腺良性肿瘤组织中的阳性率分别为100%和82.5%,均显著高于甲状腺癌组织中的45%(P<0.05),即PTEN在甲状腺癌中表达显著降低;PTEN在甲状腺乳头状癌淋巴结转移组和无淋巴结转移组阳性表达率分别为15%和60%,差异有显著性(x2=10.91,P=0.001);PTEN在甲状腺乳头状癌在包膜侵犯组和无侵犯组的阳性表达率分别为25.93%和60.61%,差异有显著性(x2=7.22,P=0.007);PTEN在甲状腺癌淋巴结转移组和包膜侵犯组的阳性表达强度显著低于无淋巴结转移和包膜侵犯组(P<0.01),有统计学意义。结论:PTEN基因表达的降低在甲状腺癌的发生和转移过程中起重要作用。更多还原

【Abstract】 Objective: To detect and analyse the expression of PTEN genes in normal thyroid,thyroid benign tumor tissue,and thyroid papillary carcinoma cancer tissue by immunohistochemical method.Meanwhile to investigate the thyroid papillary carcinoma diagnosis and treatment of significance.Methods: Adapt SP immunohistochemical method,took known positive tissue as positive control,took phosphate buffer(PBS) instead of antibodies as a negative control,all tissue were studied with HE dyeing and immunohistochemical staining.Results: In three groups,PTEN protein expressed in the organization with significant difference(P<0.001);the positive expres-sion in normal thyroid and thyroid benign tumor tissue were 100% and 82.5% respectively which were significantly higher than the 45% of thyroid cancer group(P<0.05),namely the expression in the PTEN thyroid cancer significantly reduced;the expression rate of PTEN in thyroid papillary carcinoma lymph node metastasis group and without lymph node metastasis group were 15% and 60%,signifi-cantly(the chi-square = 10.91,P = 0.001).PTEN in thyroid papillary carcinoma in coating infringed group and no violation of the group posi-tive expression rate were 25.93% and 60.61% and the difference was significantly(the chi-square = 7.22,P = 0.007).The strength of pos-itive PTEN in thyroid cancer lymph node metastasis group and the infringement of coated positive were significantly lower than lymph node metastasis and coated infringed group(P<0.01) which reached a statistical significance.Conclusion: The reducing of the expression of PTEN gene palys a key role in thyroid cancer and the process of disease transfer.更多还原