【摘要】 目的:通过STR分型,对两例卵巢囊腺癌病理标本的组织细胞进行个体识别,以鉴别其中一例病理标本癌细胞是否源于污染。方法:对甲醛固定石蜡包埋组织,分别切片后在倒置显微镜下,用显微操作系统分离出不同形态的细胞团,对提取的DNA样本进行常规STR扩增检验。结果:经过STR分型比对,证实病理标本存在污染,其中一例的癌细胞来源于另一个体。结论:STR分型是用于病理石蜡包埋组织标本污染个体识别的有效手段。更多还原

【Abstract】 Objective: To use the STR typing to identify the two pathological samples of ovary cystadenocarcinoma,in order to identify whether one of two pathological samples was contaminated.Methods: Paraffin-embedded tissues were sliced and the different morphological cells were isolated by micro-operating system on inverted microscope.Extract DNA of the isolated cells,and get the geno-type through regular PCR-STR method.Results: The genotype show the existence of contamination of the pathological samples and prove that cancer cells of sample were originated from the other sample.Conclusion: Individual identification through the PCR-STR method was the effective method to identify the contaminated pathological slice.更多还原