【摘要】 以世界主要油料作物油菜(Brassic anapus L.)为研究对象,研究了干旱胁迫下外施壳寡糖对叶片超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、叶绿素荧光参数及叶片相对含水量的影响。结果表明:壳寡糖处理早期油菜叶片SOD和POD活性明显下降,后期SOD和POD活性明显升高;壳寡糖处理后脯氨酸含量明显升高。壳寡糖还能够诱导光系统Ⅱ最大光化学效率(Fv/Fm)、光系统II有效光化学量子产量(Fv’/Fm’)、光化学荧光猝灭系数(qP)和光系统II电子传递量子产量(ΦPSⅡ)明显提高,同时初始荧光(Fo)和非光化学荧光猝灭系数(qN)明显降低。此外,壳寡糖还能够提高油菜叶片相对含水量。研究表明,外施壳寡糖改善了油菜抗旱相关的生理指标进而增强其抗旱能力。更多还原

【Abstract】 Taking Brassica napus L., an oil crop broadly cultivated in the world as test object, a greenhouse experiment was conducted to study the effects of oligochitosan on the leaf super oxide dismutase (SOD) and peroxidase (POD) activities, proline content, chlorophyll fluorescence parameters, and relative water content under drought stress. After spraying oligochitosan, the leaf SOD and POD activities decreased at early stage but increased obviously at late stage, whereas the leaf proline content increased obviously throughout the process. The maximum quantum yield of photosystem Ⅱ (Fv/Fm), excitation capture efficiency of open centers (Fv′/Fm′), photochemical quenching coefficient (qP), and effective quantum yield of photosystem II photochemistry (ΦPSII) all increased obviously, while the minimal fluorescence of dark-adapted state (Fo) and non-photochemical quenching coefficient (qN) had an obvious decrease. The leaf relative water content also increased. It was suggested that oligochitosan could improve the related physiological indices of B. napus against drought, and further, enhance the drought resistance of the oil crop.更多还原