【摘要】 用乙醇提取法提取葛根中的异黄酮,以葛根素作为标准品绘制标准曲线,用紫外分光光度法检测异黄酮含量,采用单因素实验,考察搅拌速度、提取温度、提取时间、液固比4个主要因素对葛根异黄酮得率的影响。在单因素实验基础上用正交实验进行优化工艺参数,其合适的工艺条件为:转速为200r/min、液固比为10:1、提取时间为60min、提取温度为60℃,在此条件下得出的葛根异黄酮含量为7.12%,转移率为94.5%。更多还原

【Abstract】 Ethanol extraction was used to extract isoflavones from Pueraria Lobata Ohwi.The content of isoflavones was detected by UV spectrophotometry using puerarin as standard sample to draw the standard curve.Based on single factor experiments and orthogonal experiments,the effect on the extraction rate was studied during stirring speed,temperature,extraction time and liquid-solid ratio.The optimal operation conditions are as follows:stirring speed 200 r/min,liquid-solid ratio 10:1,time 60 min,temperature 60 ℃,under this condition puerarin isoflavones extraction yield was 7.12%,transfer rate was 94.5%.更多还原