【摘要】 采用酶法水解大鲵蛋白制备蛋白粉,以水解度为指标,通过单因素试验和正交设计试验,研究了饲养大鲵蛋白的水解条件。结果表明:采用碱性蛋白酶:木瓜蛋白酶=1:3(g/g)为试验用酶,水解的最佳条件为料液比1:7、酶浓度2400 U/g、pH7.5、水解时间5 h、水解温度55℃,该条件下水解度可达36.18%。更多还原

【Abstract】 With the hydrolysis degree as the index,the conditions of enzymatic hydrolysis on proteins from Andrias davidianus were studied by single-factor and orthogonal experimen.The result shows that choosing alkaline protease:papain=1:3(g/g) as the certain enzyme,the optimum conditions of enzymolysis were the ratio of material and liquid 1:7,the enzyme concentration 2400 U/g,pH7.5,hydrolyzed for 5 h at 55 ℃.Under above conditions,the hydrolysis degree was 36.18%.更多还原