【摘要】 利用冻融循环处理结合均相脱乙酰制备了天然水溶性甲壳素(WSC)。在3种不同溶剂中,紫外光谱差异不大。脱乙酰度一定时,水溶性甲壳素样品的水溶液在208 nm~212 nm的吸光度A与波长λ呈线性关系,其斜率与对应溶液的浓度呈线性关系,初步建立了1种用紫外多波长回归测定WSC水溶性的方法。圆二色谱显示,在稀酸溶液中水溶性样品比脱乙酰度相当的非水溶性样品分子链更加伸展。冻融循环和均相脱乙酰打破了分子间强氢键相互作用,使WSC的空间结构更为舒展,较少呈现螺旋结构,更易溶于水中。更多还原

【Abstract】 A natural water-soluble chitin(WSC) was prepared by alkaline treatment under homogeneous conditions using freezing-thawing cyclic treatment.There was no difference between the ultra voilet spectroscopy in three different solvents.In aqueous water-soluble chitin solution,there was a linear relationship between absorbance(A) and wavelength(λ) in the range of 208 nm-212 nm,when the degree of deacetylation was as constant.And there was also a good linear relationship between the value of the slope and the concentration of WSC.As a result,a multiwavelength linear regression ultra voilet spectrophotometry was established for determining water solubility of WSC.The circular dichroism spectra showed that in diluted acid solution,there was more extend molecular chain of WSC than half deacetylated non-water-soluble chitin.It meaned that freezing-thawing-cylic treatment and homogeneous deacetylate broke the strong molecular hydrogen bonds,made WSC have more stretching spatial structure,less helix structure,and led it soluble in water more easily.更多还原