【摘要】 目的:研究热水浸提法从姬菇中提取姬菇多糖(PCNP)对酒精所致小鼠急性肝损伤的保护作用。方法:小鼠被随机分为空白对照组、模型组、阳性对照组(150mg/(kg.d))、多糖各剂量组(100、200、400mg/(kg.d)),连续灌胃30d后,除空白对照组外,给予50%体积分数的乙醇溶液建立小鼠急性肝损伤模型。12h后,小鼠脱臼处死后取血液和肝脏测定各项抗氧化指标,并观察肝组织病理学变化。结果:与模型组相比,多糖各剂量组能降低血清谷草转氨酶(AST)、谷丙转氨酶(ALT)活性和肝脏丙二醛(MDA)含量,提高肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量。结论:PCNP能明显改善肝组织病理损伤程度,对酒精所致小鼠急性肝损伤具有明显保护作用。更多还原

【Abstract】 The present study investigated the protective effect of polysaccharides in hot water extract from Pleurotus cornucopiae(PCNP) on alcohol-induced acute liver injury in mice.Mice were randomly divided into blank control,alcohol-induced liver injury model,positive control and PCNP treatment(at doses of 100,200 mg/(kg.d) and 400 mg/(kg.d) groups.After 30 consecutive days of intragastric administration,all the mice except for blank control group were given by gavage 50% alcohol at a dose of 12 mL/kg to induce acute liver injury.At 12 h after alcohol administration,all the mice in each group were killed by cervical dislocation and blood and liver were collected for the measurement of antioxidant indicators and pathological observation of liver tissue.Compared with the model group,PCNP administration resulted in a decrease in MDA content in the liver and the activities of AST,ALT in the serum,and an increase in the activities of SOD and CAT and GSH content in the liver of mice.In conclusion,PCNP can obviously improve pathological liver injury and protect against acute alcohol-induced liver injury in mice.更多还原