【摘要】 用95%乙醇浸泡提取传统药食两用植物牛蒡叶,并用石油醚、乙酸乙酯和正丁醇依次对浸提液进行萃取。利用DPPH自由基清除法、O2-.清除法及.OH清除法测定各萃取部位的抗氧化活性,以VC、VE和叔丁基对苯二酚(TBHQ)为对照物。结果表明:牛蒡叶乙醇提取物具有较好的体外抗氧化活性,其中乙酸乙酯萃取物(E2)和正丁醇萃取物(E3)具有较强的清除DPPH自由基的能力,且活性强于TBHQ,利用标准曲线法对乙酸乙酯萃取物和正丁醇萃取物进行绿原酸含量的测定,发现绿原酸含量分别达44.34mg/g和35.48mg/g。更多还原

【Abstract】 This study was designed to investigate the DPPH,superoxide anion and hydroxyl free radical scavenging activities and reducing power of different fractions(petroleum ether,ethyl acetate and n-butanol) of the 95% ethanol extract of burdock(Arctium lappa L.) leaves using VC,VE and TBHQ as controls.The ethanol extract of burdock leaves revealed strong antioxidant activity in vitro.Ethyl acetate and n-butanol fractions had strong DPPH radical scavenging activity and were superior to TBHQ,and the content of chlorogenic acid in the two fractions was 44.34 mg/g and 35.48 mg/g,respectively.更多还原