【摘要】 为建立胰岛素抵抗脂肪细胞模型,以3T3-L1前脂肪细胞为研究材料,通过3-异丁基-1-甲基黄嘌呤、地塞米松和胰岛素联合诱导其分化为3T3-L1脂肪细胞,通过葡萄糖消耗实验研究胰岛素诱导3T3-L1脂肪细胞产生胰岛素抵抗的作用浓度和时间关系,发现10-7mmol/L胰岛素诱导处理48h是3T3-L1脂肪细胞产生胰岛素抵抗的最适条件,该细胞模型的胰岛素抵抗状态可维持48h。通过高浓度胰岛素成功诱导3T3-L1脂肪细胞产生胰岛素抵抗,具有建模周期短、重复性好、操作简便的优点,该细胞模型可以用于抗胰岛素抵抗天然产物的筛选研究。更多还原

【Abstract】 3T3-L1 preadipocytes were differentiated into 3T3-L1 adipocytes with combined 3-isobutyl-1-methylxanthine,dexamethasone and insulin for establishing an insulin-resistant adipocyte cell model.Treatment with 10-7mmol/L insulin for 48 h was the best condition for induction of insulin resistance as determined by glucose consumption experiments.Insulin resistance could be kept in the developed model for 48 h.High concentration of insulin could successfully induce insulin resistance in 3T3-L1 adipocytes with the advantages of time saving,good repeatability and ease operation.This model will be also available for screening of natural products that improve insulin sensitivity.更多还原