【摘要】 通过检测大肠杆菌体内H+-ATP酶的活性、利用氨基酸自动分析仪测定萃取物的氨基酸成分、扫描电镜对处理前后的大肠杆菌进行观察,比较考察高压CO2对大肠杆菌菌粉和菌悬液的杀菌效果。结果表明:高压CO2杀灭大肠杆菌不是由某一机理单独作用的,而是由机械破裂致死机制、萃取机制和酸化机制共同作用的结果。更多还原

【Abstract】 The efficacies of high pressure carbon dioxide(HPCD) treatment for inactivating dried culture powder and suspension of E.coli were comparatively evaluated by measuring the activity of H+-ATPase in E.coli,determining the amino acid composition of HECD extracts using an amino acid analyzer,and observing the morphology of E.coli cells under a scanning electron microscope(SEM) before and after HPCD treatment.The results obtained demonstrated that the inactivation of E.coli by HPCD was achieved jointly by mechanical rupture,extraction and acidification rather than a single action.更多还原