【摘要】 目的:采用正交试验法,优选枣核中总黄酮的微波辅助提取工艺。方法:以芦丁为对照品,采用分光光度法进行测定,以枣核黄酮提取率作为考查指标,对影响黄酮提取工艺的因素进行研究。通过单因素试验研究微波功率、提取时间、提取温度、料液比和提取溶剂乙醇体积分数对提取效果的影响。然后,利用正交试验法优化最佳提取工艺条件。结果:对枣核黄酮提取的影响程度大小顺序为料液比>提取温度>提取时间>乙醇体积分数>微波功率,正交试验结果表明最佳提取工艺参数为料液比1:60(g/mL)、提取温度60℃、乙醇体积分数50%、提取时间20min、微波功率500W。在最佳工艺条件下,枣核黄酮粗品的提取产率为10.14%,粗品中黄酮含量为7.0 0%。结论:采用正交试验-微波提取工艺,能显著提高枣核黄酮的提取效率,具有较好的提取效果。更多还原

【Abstract】 Objective: Orthogonal array design was used to optimize the microwave-assisted extraction of total flavonoids from Chinese date seeds.Methods: Total flavonoids were spectrometrically determined using rutin as a reference compound.The effects of microwave power,extraction time,temperature,solid-to-solvent ratio and ethanol concentration on the extraction yield of total flavonoids were investigated by one-factor-at-a-time experiments.Subsequently,these extraction parameters were optimized by orthogonal array design.Results: Five extraction parameters were ranked in descending order of their effect on the extraction of total flavonoids as follows: solid-to-solvent ratio>extraction temperature>extraction time>ethanol concentration>microwave power.Their optimal levels were 1:60(g/mL),60 ℃,20 min,50% and 500 W,respectively.Under these conditions,the extraction yield of crude total flavonoids was 10.14%,and the total flavonoid content of the extract obtained was 7.00%.Conclusion: The extraction efficiency of total flavonoids from Chinese date seeds is remarkably increased by microwave extraction and orthogonal array optimization.更多还原