【摘要】 以豌豆根瘤菌(Rhizobium leguminosarum 1.1723)为出发菌株,利用紫外-LiCl和超声波进行诱变,以期获得高产辅酶Q10菌株。结果表明:1g/L LiCl诱变能显著提高菌株的正突变率,超声波诱变会引起细胞壁结构与构成发生改变。通过罗红霉素初筛和摇床复筛,获得辅酶Q10突变株C40-05,胞内辅酶Q10产量为1.198mg/g干菌,比出发菌株(0.389mg/g)提高了208%。菌株经5次传代培养,胞内辅酶Q10产量下降了2.67%,突变株遗传形状稳定,可作为辅酶Q10生产菌株。更多还原

【Abstract】 Rhizobium leguminosarum 1.1723 was mutagenized by means of LiCl treatment followed by UV irradiation or ultrasonic treatment alone to yield coenzyme Q10-producing strains.The results showed that 1 g/L LiCl could obviously increase the positive mutation rate and that ultrasonic treatment could change cell wall structure of Rhizobium leguminosarum.A mutant C40-05 with high productivity of coenzyme Q10 was obtained through preliminary screening with roxithromycin and secondary screening by means of shake flask cultivation.Its coenzyme Q10 yield was up to 1.198 mg/g,which revealed a 208% increase compared with the original stain.Only 2.67% reduction in coenzyme Q10 yield of the mutant was observed after the fifth passage.Therefore,the obtained mutant is a stable strain that deserves to be studied further.更多还原