【摘要】 采用16S rDNA基因的限制性酶切片段长度多态性分析(16S rDNA-PCR-RFLP)和16S～23S rDNA基因间区(ISR)的酶切多态性分析(ISR-PCR-RFLP)技术,研究分离自川西高原牦牛酸奶子的81株乳酸菌的遗传多样性,并结合16S rDNA序列分析技术研究16S rDNA-PCR-RFLP的各类群代表菌株的系统发育关系。结果表明:供试菌株共有23种16S rDNA遗传型,供试菌株按61%相似系数可分为4个类群,按81%相似系数,可分为16个类群,结合序列分析结果,乳杆菌属(Lactobacillus)、肠球菌属(Enterococcus)细菌为主要类群;16S～23S rDNA基因的ISR扩增产物的酶切分析表明,供试菌株共有19种ISR遗传型,按60%相似系数,供试菌株可分为4个类群,按80%相似系数,供试菌株可划分为14个类群。更多还原

【Abstract】 The genetic diversity and the phylogeny of 81 lactic acid bacterial strains isolated from yak yogurt samples collected from Western Sichuan Plateau were studied by using 16S rDNA-PCR-RFLP,ISR-PCR-RFLP techniques,and 16S rDNA gene sequence analysis.The results showed totally 23 16S rDNA genotypes were detected and the tested strains could be divided into four taxa on the basis of 61% similarity,and 16 taxa on the basis of 81% similarity.Sequence analysis of 16S rDNA genes showed that genus Lactobacillus and genus Enterococcus were two major genera in all tested strains.ISR-PCR-RFLP analysis also revealed 19 ISR genotypes,and the tested strains could be divided into four taxa on the basis of 60% similarity,and 14 taxa on the basis of 80% similarity.更多还原