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响应面法优化脐橙渣中水溶性膳食纤维提取工艺

Response Surface Methodology for Optimization of Extraction Process of Soluble Dietary Fiber from Navel Orange Residue

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【作者】 吴笑臣王科军钟金莲罗序中

【Author】 WU Xiao-chen,WANG Ke-jun,ZHONG Jin-lian,LUO Xu-zhong(College of Chemistry and Life Science,Gannan Normal University,Ganzhou 341000,China)

【机构】 赣南师范学院化学与生命科学学院

【摘要】 以赣南脐橙渣为原料,采用纤维素酶制备水溶性膳食纤维。在单因素试验基础上,选取酶解温度、加酶量(质量分数)和酶解时间为响应变量,以水溶性膳食纤维得率为响应值,利用Box-Behnken试验设计方案和响应面分析法,建立水溶性膳食纤维得率与响应变量的回归方程,并确定最佳提取条件为酶解温度48℃、加酶量1.25%、酶解时间5h,此条件下水溶性膳食纤维得率为13.11%,与预测值13.14%较为一致。

【Abstract】 Navel orange residue was treated with cellulase to extract soluble dietary fiber.Extraction parameters including extraction temperature,cellulase dose and length of extraction time were optimized using Box-Behnken design and response surface methodology based on single factor investigations for achieving maximum yield of soluble dietary fiber.A regression equation reflecting the relationship between the yield of soluble dietary fiber and the extraction parameters was set up.The optimal extraction parameters were found as follows: extraction temperature,48 ℃;cellulase dose,1.25%;length of extraction time,5 h.Under the optimized conditions,the actual yield of soluble dietary fiber was 13.11%,close to the predicted value of 13.14%.

【基金】 江西省科技厅科技支撑计划项目(2010BNB1400);江西省教育厅科学技术研究项目(GJJ10587),江西省教育厅青年科学基金项目(GJJ11214);赣州市果业基金项目(GZGY2008-1);赣南师范学院自然科学研究课题(09kyz10)
  • 【分类号】TS201.2
  • 【被引频次】26
  • 【下载频次】398
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