【摘要】 以低档茶叶提取茶多酚后的茶渣为原料,研究茶叶多糖的水提工艺及初步纯化技术。分别就提取过程中的料液比、浸提时间、浸提温度、浸提次数进行了单因素实验,并用L9(34)正交实验优化提取工艺,用醇沉及脱蛋白技术对茶多糖进行初步纯化,得出优化的工艺为:料液比1∶30,浸提温度85℃,浸提时间2h,浸提次数3次,浓缩液与95%乙醇用量1∶5,乙醇沉淀静止6h,Sevage法脱蛋白3次,茶多糖的得率为4.10%。更多还原

【Abstract】 The water extraction and preliminary purification technology of tea polysaccharide(TPS) was studied. Main factors of extraction technology such as material-to-liquid ratio,extraction time,extraction temperature,and number of extraction times were researched through single-factor experiments. The optimum extraction technology was confirmed through orthogonal test of L9(34). The preliminary purification technologies of alcohol precipitation and deproteintion on tea polysaccharide were investigated. The optimal conditions were as follows:the ratio of material to liquid was 1∶30,extraction temperature was 85℃,extraction time was 2h for three times,ratio of concentrated solution to 95% alcohol was 1∶5,alcohol quiescence time was 6h,and the protein was removed with Sevage method for 3 times. Under these conditions,the final polysaccharide yield was 4.10%.更多还原