In order to beyond the limits that current real-time PCR method could only detect group A Streptococcus,a new method was established using real-time fluorescence PCR assays with detection range consistent to the national standard GB/T4789.11-2003.Bacitracin sensitive experiment was used to validate the detection range of Streptococcus hemolyticus in the national standard GB/T4789.11.Group A,C and G Streptococcus were selected and the DNA sequences of which were aligned using CLUSTAL X software.Conserved reg...
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