【摘要】 探讨肝癌模型鼠与正常小鼠肝组织B4GalT(β-1,4-半乳糖转移酶)家族mRNA表达差异以及对细胞膜相关糖链的影响.采用RT-PCR方法检测肝癌模型鼠和正常对照小鼠肝癌组织中B4GalT家族7个成员以及唾液酸α-2,3转移酶ST3GalⅢ、ST3GalⅣ、ST3GalⅥ、α-1,6-岩藻糖转移酶FUT8 mRNA表达差异,应用凝集素芯片检测细胞膜表面半乳糖、岩藻糖、唾液酸表达情况.结果显示:与正常对照组相比,肝癌模型鼠肝组织中B4GalT-1和B4GalT-3、ST3GalⅣ和ST3GalⅥ、FUT8呈现高表达,肝癌细胞膜半乳糖、岩藻糖、唾液酸类型糖链增加,提示B4GalT-1和B4GalT-3与肝癌细胞膜半乳糖链增加相关.由于细胞Galβ-1,4-GlcNAc糖表位在ST3GalⅢ、ST3GalⅣ或ST3GalⅥ催化下与唾液酸α-2,3连接生成s-lewis x抗原前体,本实验中B4GalT-1和B4GalT-3与ST3GalⅣ、ST3GalⅤ、FUT8 mRNA表达具有相关性,提示B4GalT-1和B4GalT-3可能与ST3GalⅣ、ST3GalⅥ以及FUT4协同作用,导致肝癌细胞膜半乳糖、岩藻糖、唾液酸类型糖链增加.更多还原

【Abstract】 To investigate different expression of mRNA in B4GalT(β-1,4-Galactosyltransferase) family be-tween mouse hepatocellular carcinoma and normal liver tissues and its influence on relevant sugar chain of membrane.The different expression of mRNAs in 7 members of B4GalT family and α-2,3 sialyltransferase ST3Gal Ⅲ,ST3Gal Ⅳ,ST3Gal Ⅵ and fucosyltransferase FUT8 were detected with RT-PCR.The expression levels of galactose,fucose and sialic acid on cell membrane surface were analyzed by lectin array.The re-sults showed that compared with normal mouse tissues,B4GalT-1,B4GalT-3,ST3Gal Ⅳ,ST3Gal Ⅵ and FUT8 in mouse hepatocellular carcinoma were identified highly expressed.Galactose,fucose and sialic acid on cell membrane surface were increased,indicating that B4GalT-1 and B4GalT-3 are related with the increasing of the galactose epitope.Because sialic acids were added to Galβ-1,4-GlcNAc epitopes to form slewis x anti-gen precursor by ST3Gal Ⅲ,ST3Gal Ⅳ or ST3Gal Ⅵ,the relationship between the mRNA expression of B4GalT-1 and B4GalT-3 with ST3gGal Ⅳ,ST3Gal Ⅴ and FUT8 in the experiment indicates that B4GalT-1 and B4GalT3 may effect synergistically with ST3Gal Ⅳ,ST3Gal Ⅵ and FUT4,which results in the increase of galactose,fucose and sialic acid on hepatocellular carcinoma cell membrane.更多还原