【摘要】 目的:探讨不同形式游泳运动后大鼠延髓腹外侧区c-fos的表达及其时效性分布规律。方法:选取正常成年雄性SD大鼠55只,随机分为对照组(5只)、持续游泳运动组(25只)和间歇游泳运动组(25只)。运动组进行6周游泳训练建立运动模型。运动结束后0、0.5、1、2、4 h等不同时间点取材,免疫组化SABC法检测延髓腹外侧区c-fos阳性神经元数目的变化。结果:(1)延髓腹外侧区头端(RVLM)持续组大鼠c-fos阳性神经元数目在运动结束后逐渐增多,至0.5 h达高峰,之后缓慢回落,至2 h后接近对照组水平。间歇组运动后即刻即可见c-fos阳性神经元数目显著增高至峰值,之后维持于较高的水平范围内呈现缓慢下降趋势,至4 h恢复至对照水平;(2)延髓腹外侧区尾端(CVLM)持续游泳运动组大鼠c-fos阳性神经元数目在运动结束后略有增加(P>0.05),0.5 h迅速下降至低于对照组水平(P<0.05),之后重新回升至对照水平,并逐渐降低。间歇游泳运动组,运动后c-fos阳性神经元数目缓慢上升,至1 h达高峰,之后回落至2 h后再次略微升高。结论:不同形式游泳运动可引起大鼠延髓腹外侧区c-fos信号通路不同程度的活化。更多还原

【Abstract】 Objective: To explore the expression of c-fos and the chronergy distribution pattern in ventrolateral medulla(VLM) of rats that treating with different kinds of swimming exercises.Methods: Fifty-five adult male SD rats were randomly divided into control group(n=5),endurance swimming group(n=25) and interval swimming group(n=25).The rats in the latter two groups were trained for 6 weeks.The brains were removed at the moments of 0,0.5,1,2,4 h after swimming,the changes of the number of c-fos immunopositive neurons in VLM were detected by immunohistochemical SABC techniques.Results:(1) In the endurance swimming group the number of c-fos immunopositive neurons increased gradually and reached to the top at 0.5 h,then reduced to the level that was equal to the control group until 2 h at rostral ventrolateral medulla(RVML).And the number of c-fos immunopositive neurons in interval swimming group peaked at 0 h,remained at the high level and turned down slowly until 4 h to the control level at RVML;(2) In the endurance swimming group,the number of c-fos immunopositive neurons showed a nonstatistically increase(P>0.05) and then reduced quickly at 0.5 h,then it increased again to the control level and reduced gradually in caudal ventrolateral medulla(CVLM).Moreover,the number of c-fos immunopositive neurons in interval swimming group peaked at 1 h,turned down until 2 h,and then increased again at CVLM.Conclusion: The different kinds of swimming exercises can induce the different activation level of c-fos signal pathway in VLM of rat.更多还原