【摘要】 为探讨MCM3和Ki-67在甲状腺乳头状癌、结节性甲状腺肿及正常甲状腺组织中的表达及意义。采用通过逆转录聚合酶链反应(RT-PCR)检测MCM3在甲状腺乳头状癌、结节性甲状腺肿及正常甲状腺组织中的表达的方法,用免疫组织化学Sp法测定MCM3和Ki-67在甲状腺乳头状癌、结节性甲状腺肿及正常甲状腺组织中的表达。结果显示:(1)DRT-PCR:MCM3与Ki-67在正常甲状腺、癌、结节性甲状腺肿中均有表达。(2)免疫组化:MCM3在正常甲状腺、癌、结节性甲状腺肿组中表达水平有差异(P<0.001),癌和结节性甲状腺肿组与正常甲状腺组表达水平有差异(P<0.01 25);Ki-67在结节性甲状腺肿组和正常甲状腺组中表达尚无差异(P>0.0125),余两两比较表达水平有差异(P<0.0125);MCM3与Ki-67在甲状腺癌组和结节性甲状腺肿组中表达水平有差异(P<0.001)。由此可知,MCM3可作为一种新的甲状腺乳头状癌标记物,较Ki-67更为可靠,从而为判断甲状腺良恶性病变提供一条新的路径。更多还原

【Abstract】 To explore the significance and expression of MCM3 and Ki-67 in normal thyroid,carcinoma and nodular goiter.RTPCR was employed to examine the level of MCM3 and Ki-67 in the normal thyroid tissue,papillocarcinoma,and nodular goiter. SP immunohistochemical technique was used to observe the expression of MCM3 and Ki-67.Results:(1) RT-PCR:Both MCM3 and Ki-67 were expressed in the normal thyroid tissue,papillocarcinoma,nodular goiter.(2) SP:The expression of MCM3 in the normal ihyroid tissue,thyroid papillocarcinoma and nodular goiter was statistically significant(P<0.001).The expression level of IVICiMS in thyroid papillocarcinoma and nodular goiter compared with the expression level in the normal thyroid tissue was statistically significant(P<0.0125).The expression of Ki-67 had no significant difference between normal thyroid tissue and nodular goiter(P>0.0125),others had significant difference(P<0.0125).The expression of MCM3 and Ki-67 had significant difference in carcinoma and nodular goiter(P<0.001).MCM3 may be used as a new marker of thyroid carcinoma,more reliable than Ki-67.offering a new approach to differentiate benign and malignant lesions of thyroid.更多还原