Objective To establish hepatitis B virus(HBV)X gene eukaryotic expression vector and identify its expression.Methods Polymerase chain reaction(PCR)was used to amplify X gene sequence,and the pcDNA6(+)vector and X gene PCR product double enzyme digestion transformed into Escherichia coli DH5 alpha.The positive clones were screened,and the double enzyme digestion and DNA sequencing were used to establish HBV X gene eukaryotic expression vector pcDNA6(+)-HBx.By polyetherimide(PEI)method,the transformation into...
【更新日期】
2013-02-01
【分类号】
R373
【正文快照】
乙型肝炎是一种高发传染病,乙型肝炎病毒(hepatitis B virus,HBV)感染后有5%～10%的患者将发展为慢性肝炎(chronic hepatitis,CH),而这又是肝细胞癌(hepatocellular carcinoma,HCC)发生的高危险因素(几率比正常人大100倍)[1-2]。HBV基因具有4个开放式阅读框架(open reading fra
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