Objective To obtain the myofibrillogenesis regulator 1S(MR-1S)-His recombinant protein through the expression of MR-1S gene and prepare anti-MR-1S antiserum after purification.To identify the stable system to preserve the recombinant protein,and to provid the reference for the investigation of MR-1S protein characteristics and biological function.Methods The recombinant plasmid pET21a-MR-1S was constructed with pronucleus expression vector pET 21a(+) and transferred into Escherichia coli BL21(DE3),and the e...