【摘要】 目的观察二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)对脂多糖(LPS)刺激大鼠肾小球系膜细胞(GMC)表达基质金属蛋白酶(MMP)-2、MMP-9和金属蛋白酶组织抑制因子(TIMP)-1、TIMP-2以及转化生长因子-β1(TGF-β1)的影响。方法大鼠GMC与EPA、DHA(10,100μmol/L)作用48 h后,采用实时定量PCR方法检测MMP-2、MMP-9、TIMP-1和TIMP-2的mRNA表达,明胶酶谱法测定MMP-2、MMP-9活性,ELISA法测定TGF-β1含量。结果 EPA、DHA可以影响MMP、TIMP的表达,改善LPS诱导的MMP活力的降低,降低TGF-β1分泌。结论EPA、DHA可以通过改善MMP、TIMP失衡、减少TGF-β1分泌等机制,对损伤的GMC起保护作用。更多还原

【Abstract】 Purpose To investigate the effects of eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) on lipopolysaccharide(LPS) induced expression of matrix metalloproteinase-2,9(MMP-2,9),tissue inhibitor of metalloproteinases-1,2(TIMP-1,2) and transforming growth factor-β1(TGB-β1) by LPS stimulated rat glomerular mesangial cells(GMCs).Methods GMCs were incubated with EPA or DHA(10,100 μmol/L) for 48 hours,and the expression of MMP-2,9 and TIMP-1,2 mRNA was measured by real-time PCR method,the gelatinase activities were detected by gelatin zymography,and TGF-β1 was measured by ELISA.Results EPA and DHA could decrease the expression of MMPs and TIPMs by LPS stimulated GMCs,but increase the ratios of MMPs/TIPMs,and activities of MMPs.EPA and DHA could also decrease the production of TGF-β1.Conclusion The data suggest that EPA and DHA may protect GMCs by regulating the imbalance of MMPs and TIMPs,and reducing the production of TGF-β1.更多还原