【摘要】 目的考察粒细胞巨噬细胞集落刺激因子(Gm-csf)对乙型肝炎疫苗免疫原性及诱导的细胞免疫效果的加强作用。方法将Gm-csf和乙型肝炎疫苗分别采用4种不同方式免疫BALB/c小鼠,免疫后,制备脾细胞,再以相应的HBsAg体外刺激,酶联免疫法(ELISA)测定细胞因子分泌水平,MTT法测定脾细胞对抗原刺激指数;以YAC-1细胞为靶细胞,测定细胞毒性T淋巴细胞活性;同时ELISA法测定血清中特异性抗体IgG1和IgG2a。结果GM+疫苗组小鼠脾细胞产生的IFN-γ水平最高,显著高于其它组(P<0.05),而各免疫组IL-5水平没有显著差别(P>0.05);GM+疫苗组小鼠脾细胞对抗原刺激的刺激指数显著高于其它各组(P<0.05);GM+疫苗组小鼠脾细胞对YCA-1的杀伤性显著高于其它各组(P<0.01)。结论通过Gm-csf的联合免疫,可提高乙型肝炎疫苗的免疫原性,增强乙型肝炎疫苗诱导的细胞免疫水平。更多还原

【Abstract】 Purpose To investigate the enhanced cellular immunity responses induced by Granulocyte-macrophage colony stimulating factor(Gm-csf)cooperated with hepatitis B vaccine in BALB/C mice.Methods All BALB/C mice were immunized with hepatitis B vaccine and Gm-csf in four different ways,respectively.Splenic lymphocytes of mice were prepared after immunization.The cytokines concentration of IFN-γ and IL-5 were detected by ELISA and the stimulation indexes were detected by MTT after stimulation with HBsAg in vitro.CTL activity was tested in against YAC-1.Then we examined the specific antibAies of sera(IgG1 and IgG2a).Results The concentration of IFN-γsecreted by mice immunized with "GM+vaccine" group was significantly higher than that of other groups(P<0.05).And there was no significant difference of the IL-5 level(P>0.05).The stimulation index of "GM+vaccine" group was significantly higher than that of other groups(P<0.05).The specific cytolytic activity of splenocyte of mice immunized with "GM+vaccine" group was the highest(P<0.01).Conclusion The immunogenicity of hepatitis B vaccine and cellular immunity responses induced by hepatitis B Vaccine could be improved by Gm-csf.更多还原