【摘要】 目的研究抗坏血酸(ascorbic acid,AA)和三氧化二砷(arsenic trioxide,As2O3)抗人骨肉瘤细胞MG-63的体外疗效。方法以MG-63细胞为体外模型,用62.5μmol/L AA与1μmol/L As2O3单独或联合处理细胞,利用新型连续活细胞图像采集和分析平台(continuous live cell imaging and analysis platform,Cell IQ)实时观察细胞的生长情况和形态学的变化。结果 1μmol/L As2O3和62.5μmol/L AA单独处理都可抑制MG-63细胞的生长并诱导细胞死亡。1μmol/L As2O3和62.5μmol/L AA联合处理细胞较单独处理组细胞抑制效果更明显。结论 AA和AS2O3抗人骨肉瘤细胞Mg-63可起到协同作用,这一结果为临床治疗骨肉瘤提供了新的思路和实验依据。更多还原

【Abstract】 Objective To study the synergistic effect of human osteosarcoma cell line MG-63 treated with ascorbic acid(AA) and arsenic trioxide(As2O3) in vitro.Methods We used continuous live cell imaging and analysis platform(cell IQ) to observe cell proliferation and morphologic change of MG-63 cells which were treated with AA(62.5 μmol/L) and As2O3(1 μmol/L) alone or together.Results MG-63 cell proliferation was depressed observed when treated by AA(62.5 μmol/L) and As2O3(1 μmol/L) independently.The effect of AA(62.5 μmol/L) plus As2O3(1 μmol/L) was synergistic,which further inhibits MG-63 cell proliferation.Conclusions The treatment of AA combined with As2O3 can induce synergistic effect on the depression of MG-63 cell proliferation.This result provides a new pathway and basic reaserch data of treating osteosarcoma in clinical practice.更多还原