【摘要】 目的 基于心率变异性(heart rate variability, HRV)分析评估不同因素致亚健康大鼠自主神经功能的影响，确定HRV指标与亚健康状态的发生关系。方法 将42只雄性SPF级Wistar大鼠，按体质量随机分成7组，即多因素组、多因素干预组、热水游泳组、饮食限制组、睡眠不足组、单纯束缚组和正常对照组，每组6只，通过热水游泳、饮食限制、睡眠剥夺、束缚等方式建立亚健康大鼠模型，多因素干预组给予300 mg/kg的抗衰老片浸膏粉，并分别在造模结束后及恢复3 d时遥测大鼠的心电图，利用HRV分析评估不同因素致亚健康大鼠自主神经功能情况。结果 与正常对照组比，时域分析显示亚健康大鼠的SDNN、RMSDD、STV、LTV均有所升高，且频谱分析显示亚健康大鼠VLF、LF、TP、LF/HF、LFnu上升和HFnu下降，且给予抗衰老片后能明显降低LF、LF/HF和LFnu(P<0.05)和显著升高HFnu(P<0.05),单因素logistic回归分析显示RRI、SDNN、LTV、LF、TP、LF/HF、LFnu、HFnu与亚健康产生密切相关(P<0.05),且经逐步logistic回归分析结果显示低下的HFnu(OR值=0.566,P=0.023)可作为评价亚健康产生的独立危险因子。结论 不同因素致亚健康大鼠的自主神经功能均出现明显的紊乱,且迷走神经活动抑制是亚健康产生的独立危险因素,可见基于HRV分析技术可无创性的客观评估机体亚健康状态的程度。更多还原

【Abstract】 Objective To assess autonomic nervous function in sub-health rats induced by different factors based on Heart Rate Variability(HRV) analysis, and determine the relationship between HRV indexes and sub-health state. Methods Fourty-two male SPF Wistar rats were randomly divided into seven groups: multi-factor group(MF), multifactor treatment group(MFT), warm swimming group(WS), dietary restriction group(DR), sleep deficiency group(SD), purely constraint group(PC) and control group(C), six animals in each group. Using warm swimming, dietary restrictions, sleep deprivation and constraint to establish sub-health rat model. Rats were daily orally administered 300 mg/kg antiaging tablet in MFT group. The ECG was monitored at the end of the modeling and recovery of 3 days,and HRV analysis has been used to investigate the autonomic nervous function in sub-health rats induced by different factors. Results Compared with control group, standard deviation of the mean R-R interval(SDNN), the root-mean-square of successive differences(RMSSD), short term variability(STV)and long term variability(LTV) parameters of their HRV time domain analysis in sub-health rats were significantly higher than those of control group, and very low frequency power(VLF), low frequency power(LF), total power(TP), LF/HF ratio, normalized low frequency(LFnu) parameters of their HRV frequency domain analysis were increased, while normalized high frequency(HFnu) was decreased.Anti-aging tablets treatment could significantly reduce the LF, LF/HF ratio and LFnu(P<0.05) and increase HFnu(P<0.05). Univariate logistic regression analysis showed that the closely relationship between RRI, SDNN, LTV, LF, TP, LF/HF ratio, LFnu, HFnu and sub-health state(P<0.05), and stepwise logistic regression analysis showed that the low HFnu(OR, value=0.566, P=0.023) maybe independent risk factors for inducing sub-health. Conclusion The autonomic nervous function in sub-health rats induced by different factors was apparent disorder, and inhibition of vagus nerve activity maybe an independent risk factor for sub-health. So HRV analysis could assess the sub-health state.更多还原