【摘要】 目的 探讨邻苯二甲酸二丁酯（DBP）对亲代（F₀）、子代（F₁）斑马鱼的生殖毒性。方法 将48对成年斑马鱼随机分为4组:空白对照组、溶剂对照组、625μg/L DBP低剂量组和1 250μg/L DBP高剂量组。连续染毒DBP 30 d后，将各组F₀代斑马鱼交配，记录产卵数、受精率以及72 h孵化率。将F₀和F₁代斑马鱼在清水中饲养180 d，进行F₁代斑马鱼交配试验，且将F₁代DBP高剂量组与对照组斑马鱼进行置换交配，记录产卵数、受精率及孵化率。进行F₀代和F₁代斑马鱼性腺组织病理学检查，并测定卵黄蛋白原（VTG） mRNA的表达水平。结果 与对照组和DBP低剂量组相比，DBP高剂量组F₀代和F₁代斑马鱼的生殖能力显著下降；置换交配实验显示DBP对雄性斑马鱼的生殖能力影响更为显著。组织病理学检查显示F₀代雄鱼精巢中精子数量减少，间质细胞稍有增多，精母细胞增多。而F₁代雄鱼精巢发育受到明显抑制，出现生精小管畸形，精子数量明显减少，以及间质细胞增生。RT-PCR结果显示DBP各剂量组F₀代和F₁代雄性斑马鱼VTG基因无明显表达，提示DBP对斑马鱼无雌激素样效应。结论 DBP高剂量组染毒可影响F₀代和F₁代斑马鱼的正常发育，使产卵量减少，受精率显著下降。F₀代和F₁代雄性斑马鱼生精小管畸形、精巢中精子数量减少与间质细胞增生同时存在。更多还原

【Abstract】 Objective To study the reproductive toxicity effects of dibutyl phthalate（DBP） on F₀（parent） and F₁（parent） zebrafish. Methods Fourty-eight pairs of adult zebrafish were randomly divided into 4 groups: blank control group, 0.01% acetone solvent control group, 625 μg/L DBP low-dose group, and 1250 μg/L DBP high-dose group. After 30-day exposure, F₀ zebrafish in each group were mated and the number of eggs, fertilization rate and 72-hours hatchability were recorded. After being kept for 180 days in clean water, F₀ and F₁ zebrafish in each group were again mated, and the replacement mating test were conducted. The Vetellogenin（VTG） mRNA of F₀ and F₁ zebrafish was measured by RT-PCT, and gonad histopathology was performed. Results The fertilization of F₀ and F₁zebrafish in 1250 μg/L DBP group was significantly lower than that in blank control or in 625 μg/L DBP group; histological examination showed that DBP slightly inhibited ovarian development of the F₀ and F₁zebrafish and slightly increased the cortical vesicular stage oocyte（Coc） and perinucleolar stage oocyte（Poc）. In F₀ male zebrafish of 1250 μg/L DBP group, the sperm count in the testes was reduced, and the spermatocyte（sc） and Leydig cells were slightly increased. In F₁ male zebrafish of 1250 μg/L DBP group, the development of testes was inhibited significantly, manifested as the co-existence of abnormal seminiferous tubules, reduced sperm count and Leydig cell hyperplasia. Exposure to DBP could not induce the expression of VTG mRNA in either F₀ or F₁ male zebrafish, indicating that DBP has no estrogen-like effects on zebrafish. Conclusion Exposure to 1250 μg/L DBP can induce reproductive toxicity in F₀ and F₁ zebrafish. The number of eggs and egg hatchability were reduced. Abnormal seminiferous tubules, reduced sperm count as well as Leydig cell hyperplasia were found.更多还原