【摘要】 目的研究三氧化二砷(As2O3)术前短期体内化学治疗对大肠癌细胞分子生物学作用机制,评价其体内化学治疗效果,为临床治疗大肠癌提供理论依据。方法选择消化内镜检查及病理活组织检查诊断为大肠腺癌且计划进行手术切除的患者30例,其中男性17例,女性13例;年龄38～73岁,平均年龄55.1岁。随机分为试验组和空白对照组,每组15例,保存患者术前行内镜诊断时所取得的癌组织。试验组:As2O3(亚砷酸)注射液10 mL加入5%葡萄糖注射液500 mL中,给予患者术前每天1次静脉滴注,连用3 d;空白对照组:不用药。两组均于第4日复查血常规和肝、肾功能后手术。取术后病理癌组织。将患者术前与术后所取标本进行如下处理:固定、包埋、苏木精-伊红染色,免疫组织化学法检测突变型P53蛋白、血管内皮生长因子(VEGF)、nm23蛋白表达情况。结果As2O3体内化学治疗可以使大肠癌细胞P53蛋白、VEGF蛋白的表达下调,差异具有显著统计学意义(P<0.01);As2O3体内化学治疗可以使大肠癌细胞nm23蛋白表达率增加,差异具有显著统计学意义(P<0.01)。结论As2O3短期体内化学治疗有抑制大肠癌细胞转移的作用。更多还原

【Abstract】 Objective To investigate the effect of short-term preoperative chemotherapy with arsenic trioxide on colorectal cancer and the underlying mechanisms,and provide clinical evidence for the treatment of colorectal cancer with arsenic trioxide.Methods A total of 30 patients were enrolled,who had been finally diagnosed as colorectal adenocarcinoma by endoscopy and biopsy and prepared for surgery,male 17,female 13,aged 38-73 years old,mean age 55.1 years old.The patients were divided into 2 groups randomly: the test group and blank control group,15 patients per group.The malignant tissues obtained by preoperative endoscopy and biopsy were conserved.The patients in the test group were intraveneously administered with 500 mL of 5 % glucose containing 10 mL of arsenic trioxide per day for 3 days while the patient in the control group received no treatment.The routine blood test,liver and renal functions were checked at the 4th day after chemotherapy and subsequently received surgery.The malignant tissues were collected during the operation,fixed,embedded,and stained with hematoxylin-eosin(HE).The expression of mutant P53 protein,vascular endothelial growth factor(VEGF) and nm23 protein were determined by immunohistochemical examination.Results It showed that chemotherapy with arsenic trioxide could down regulate the expression of P53 and VEGF,and up regulate the expression of nm23 protein in the colorectal cancer tissues.The differences in the expression of the proteins between the test group and control group were statistically significant(P < 0.01).Conclusion It is demonstrated that short-term chemotherapy with arsenic trioxide could inhibit the metastasis of colorectal cancer.更多还原