【摘要】 目的观察阻断肾素—血管紧张素—醛固酮系统不同环节对肝纤维化大鼠肝组织的影响。方法取6周龄SD大鼠50只,随机分为正常组、模型组、卡托普利组、氯沙坦组和螺内酯组,各10只。正常组皮下注射橄榄油,余大鼠予40%CCl4腹壁皮下注射,3 mL/kg,首剂加倍,1次/3 d,制备大鼠肝纤维化模型;次日,卡托普利组用卡托普利60 mg/kg、氯沙坦组用氯沙坦10 mg/kg、螺内酯组用醛固酮受体拮抗剂螺内酯100 mg/kg、模型组和正常组用等量生理盐水灌胃,1次/d。均于第8周处死各组动物,取肝组织,予HE染色和Masson染色。结果模型组、卡托普利组、氯沙坦组、螺内酯组肝纤维化程度均高于正常组(P均<0.05);卡托普利组、氯沙坦组、螺内酯组肝纤维化程度相近(P均>0.05),均较模型组明显好转(P均<0.05)。结论阻断肾素—血管紧张素—醛固酮不同环节具有相同的抑制大鼠肝纤维化的作用。更多还原

【Abstract】 Objective To observe the effects on hepatic histopathology of hepatic fibrosis in rats after local blockage of renin-angiptensin-aldosterone system at different levels.Methods Fifty SD rats aged 6 weeks were randomized into five groups,which were normal control group,model group,captopril group,losartan group and spironolactone group,with 10 rats in each.In the normal group,the rats received subcutaneous injection of olive oil subcutaneous injection of olive oil,while other rats were received subcutaneous injection of CCl4-induced hepatic fibrosis.Next day,captopril group received intragastric instillation daily with captopril 60 mg/kg,losartan group received intragastric instillation daily with losartan 10 mg/kg,and spironolactone group received intragastric instillation daily with spironolactone 100 mg/kg.Control group and model group received normal saline everyday.Each group was sacrificed at 8 weeks.Hepatic fibrosis was evaluated by Hematoxylin-eosin staining and Masson staining.Results The degree of model group,captopril group,losartan group and spironolactone group hepatic fibrosis were higher than that in normal group(P<0.05).The degree of captopril group,losartan group and spironolactone group hepatic fibrosis were similar(P>0.05),and lower than that in the model group(P<0.05).There were no significant differences among the three treatment groups.Conclusion The blockage of renin-angiptensin-aldosterone system at different sites have the same inhibition of hepatic fibrosis.更多还原