【摘要】 目的探讨糖尿病大鼠肾组织中血管内皮生长因子(VEGF)的表达,以及雷帕霉素对其表达的影响。方法将SD大鼠随机分为正常对照组(A组)、糖尿病组(B组)和雷帕霉素组(C组),用链脲佐菌素(STZ)诱导SD大鼠制备糖尿病模型。治疗8周后处死大鼠,采用RT-PCR法检测3组大鼠肾脏组织中VEGF RNA表达,Western印迹法检测VEGF蛋白表达,同时测定大鼠尿白蛋白/肌酐、平均肾小球体积、肾质量指数等。结果 B组和C组大鼠肾组织中VEGF的表达水平均较A组升高(P<0.05),而C组VEGF的表达水平低于B组(P<0.05);VEGF的表达水平与尿白蛋白/肌酐、平均肾小球体积呈正相关(P<0.05)。结论 VEGF参与糖尿病大鼠肾脏损害的发生,雷帕霉素可以通过下调VEGF的表达发挥肾脏保护作用。更多还原

【Abstract】 Objective To investigate the expression of vascular endothelial growth factor(VEGF) in the renal tissue of diabetic rats and the effects of rapamycin on their expression.Methods SD rats were randomly divided into control group(group A,n=8),diabetic nephropathy group(group B,n=8) and rapamycin group(group C,n=8).We induced diabetic rat model by a single intraperitoneal injection of streptozotocin(STZ,60 mg/kg).The rats were sacrificed at 8 weeks,the urine sample in the last 24 hours was collected for analysis of albuminuria and creatinine.The expressions of VEGF in the renal tissue were assessed using reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Urine albumin/creatinine ratio(ACR),kidney weight index(KWI) and mean glomerular volume(GV) were observed.Results The expression of VEGF increased in the renal cortex in group B and group C compared to group A(P<0.05),and the expression of VEGF in group C was lower significantly than that in group B.The expression of VEGF was correlated positively with urine albumin/creatinine ratio and mean glomerular volume(P<0.05).Conclusions VEGF plays an important role in the development of early diabetic nephropathy.Rapamycin may exert a renal protection on diabetic nephropathy,which mechanism may be possibly partly through the reduction of expression of VEGF in kidney.更多还原