【摘要】 目的培养人肝癌细胞系MHCC-97H中具有干细胞特性的细胞群,探讨其分子生物学特点。方法将人肝癌细胞系MHCC-97H分别培养于干细胞培养体系及普通培养体系(分别为观察组及对照组)。培养10 d后显微镜(100倍及400倍)观察细胞生长情况;免疫荧光法检测细胞表面蛋白CD133、细胞核相关抗原(Ki67)、基质金属蛋白酶9(MMP9)、多药耐药蛋白1(MDR1)表达;West-blot法检测相应蛋白表达。结果观察组细胞呈半贴壁悬浮生长的克隆球;对照组细胞呈贴壁生长。观察组CD133、MDR1、MMP9呈高表达,对照组未见表达,P<0.05;两组Ki67均呈高表达。结论 MHCC-97H中存在一定数量的肿瘤干细胞,该群细胞是介导肝癌发生转移和耐药的主要因素。更多还原

【Abstract】 Objective To culture the human liver cancer lines MHCC-97H with stem cell characteristics in vivo,and to investigate its biology behavior.Methods Cultured the human liver cancer cells in serum-free medium and normal medium respectively(observed group and control group respectively).Ten days later,microscope was used to detect the cell growth state,immunofluorescence and West-blot assay were carried out to display the protein of CD133,MMP3,Ki67,MDR1 expression level.Results The cells in the observed group grown in the way of semi-adherent floating tumor spheres;while which in the control group grown in the way of adhesion.CD133,MDR1,MMP9 expressed highly in the observed group,but no expression were found in the control group(P<0.05);Ki67 expressed highly in the two groups.Conclusion There exist a subpopulation of cells with characteristic of stem cell in human liver cancer cell MHCC-97H line,which maybe the main factor that mediated the metastasis and drug-resisitance of the hepatic carcinoma.更多还原