【摘要】 目的探讨三七总皂苷(PNS)治疗百草枯(PQ)中毒肺损伤的效果及机制。方法将150只SD雄性大鼠分为对照组(30只)、PQ组(60只)及PNS组(60只)。PQ组和PNS组一次性予PQ 25 mg/kg灌胃染毒,制作PQ中毒模型,对照组予等体积生理盐水灌胃。PNS组于染毒前15 min阴茎静脉注射PNS 50 mg/kg,以后每日1次给药直至处死前;PQ组、对照组分别在同时间点阴茎静脉注射等体积生理盐水。观察各组染毒后6 h、12 h、1 d、3 d、5d、7 d肺组织病理改变,检测血清IL-1β水平、肺泡灌洗液中PMN的NF-κB p65表达和中性粒细胞凋亡率。结果对照组肺组织结构基本完整,PQ组炎性细胞浸润明显增多,肺组织损伤程度加重,PNS组肺组织损伤程度较轻。PQ组IL-1β水平与NF-κB p65水平明显高于对照组、明显低于PNS组(P均<0.01)。2 d时PQ组PMN凋亡率明显低于对照组,PNS组相应时间点明显高于PQ组(P均<0.05)。结论三七总皂苷能减轻百草枯中毒导致的肺组织损伤,其机制为降低肺组织中IL-1β与NF-κB水平。更多还原

【Abstract】 Objective To investigate the effect of panax notoginseng saponins(PNS)on the paraquat poisoned lung injury and its mechanism.Methods Total of 150 male SD rats were randomly divided into 3 groups: normal control(C) group(30 rats),PQ group(70 rats) and PNS group(70 rats).The PQ and PNS groups were given paraquat by gastric perfusion(25 mg/kg);The C group was given saline by gastric perfusion.The PNS group was protected by intravenous injections of penis with PNS(50 mg/kg)15 mins before exposure of paraquat,once a day until killed.The PQ and C groups were given saline.Histological changes of lung tissues were observed at different time of 6 h,12 h,1 d,3 d and 7 d after poisoned.The level of IL-1β in serum,the expression of NF-κB P65 in PMN of BALF and the ratio of PMN apoptosis were detected.Results The lung tissue structure of C group was basically integrate,and which of the PQ group showed more infiltration of inflammatory cells and serious injury,the injury of the PNS group was slighter.The level of IL-1β and NF-κB p65 in PQ group were significantly higher than those in C roup and lower than those in PNS group(all P<0.01).The ratio of PMN apoptosis at 2 d in PQ group was lower than those in C group and PNS group(all P<0.05).Conclusions PNS may alleviate lung injury in acute paraquat poisoned rats by inhibiting the expression of IL-1β and NF-κB P65.更多还原