【摘要】 目的探讨宫腔粘连(IUA)的发生与结缔组织生长因子(CTGF)和转化生长因子-β1(TGF-β1)的关系,为临床预测和预防术后宫腔再粘连提供理论依据。方法以我院行宫腔镜IUA分离术的中重度IUA患者59例为实验组,正常子宫内膜48例为对照组。采用免疫组化法检测CTGF和TGF-β1在IUA患者的子宫内膜、粘连组织及正常子宫内膜组织中的表达。术后1、3个月进行宫腔镜检查,随访IUA患者的月经改善和宫腔情况。结果 CT-GF和TGF-β1在正常子宫内膜、IUA的子宫内膜和粘连组织中均有表达,阳性表达率分别为75.00%和78.80%、80.60%和82.20%、25.93%和74.07%,CTGF和TGF-β1的表达在分泌期子宫内膜高于增殖期子宫内膜;中重度IUA患者的子宫内膜中CTGF和TGF-β1的平均阳性积分高于正常子宫内膜(P<0.01);CTGF与TGF-β1在IUA子宫内膜中的表达呈正相关(r=0.452、P<0.05)。结论 CTGF与TGF-β1参与了IUA的发生发展,且CTGF与TGF-β1的表达有相关性。更多还原

【Abstract】 Objective To explore the relationship between the expression of connective tissue growth factor(CTGF) and transforming growth factor-beta 1(TGF-β1) and the formation of intrauterine adhesion(IUA).Methods Fifty-nine women with moderate or severe IUA as the study group and forty-eight women with normal endometrium as the control group,all cases underwent hesteroscopic operation in Fuxing hospital.The expression of CTGF and TGF-β1 in the tissues of IUA and the normal endometrium were measured by immunohistochemistry method.The improvement of menstruation and recurrent adhesion were followed up.Results Positive expression of CTGF and TGF-β1 were found in the normal endometrium,the endometrium of IUA patients and the adhesive tissue with rates of 75.00% and 78.80%,80.60% and 82.20%,25.93% and 74.07%,respectively.The positive expression of CTGF and TGF-β1 in the secretory endometrium were higher than those in the proliferative endometrium.The average positive integral of CTGF and TGF-β1 in the endometrium of IUA patients were higher than those in the normal endometrium with significant difference(P<0.01).The analysis of immunohistochemistry results in the endometrium of IUA patients showed positive correlation between the expression of CTGF and TGF-β1(r=0.452,P<0.05).Conclusions The expression of CTGF and TGF-β1 are correlated with the occurrence and development of IUA.The expression of CTGF was correlated with that of TGF-β1 in the endometrium of IUA.更多还原