【摘要】 目的探讨贝那普利、替米沙坦对体外原代培养的人网膜和皮下来源的前脂肪细胞脂联素(APN)表达的影响。方法自12例行电切开腹部手术的健康成年女性腹部皮下和网膜分离前脂肪细胞,分为3组,即无干预正常对照组(NC组)、血管紧张素转换酶抑制剂类药物贝那普利组(ACEI组)和血管紧张素Ⅱ受体拮抗剂类药物替米沙坦组(ARB组),诱导分化共14 d。放射免疫法测定各组APN mRNA表达水平。结果与NC组比较,ACEI组、ARB组体外原代培养的人网膜和皮下前脂肪细胞中APN mRNA表达明显增强。结论贝那普利、替米沙坦可促进网膜来源的前脂肪细胞的分化,在以腹型肥胖为特征的代谢综合征干预方面可能具有广泛的应用前景。更多还原

【Abstract】 Objective To investigate the effects of Benazapril and Telmisartan on the adiponectin expression of human preadipocytes.Methods Human omental and subcutaneous preadipocytes were isolated from abdominal adipose tissue obtained from 12 healthy adult women undergoing elective abdominal surgery.Preadipocytes were difierentiated for 14 days without any treatment(Group NC)or in the presence of either angiotensin convening enzyme inhibitor Benazapril(Group Benazapril),angiotensin Ⅱ receptor blocker Telmisartan(Group Telmisartan).The approach of radioimmunoassay was used to detect the adiponeetin expression level.Results Benazapril and Telmisartan can increase the differentiation,improve the expression and protein excretion levels in human primary culture omental and subcutaneous preadipocytes in vitro.Conclusion Telmisartan and Benazapril have stronger effects on human omental preadipocytes in adiponectin mRNA expression and secretion.This may suggests a wide perspective of RAS blockers on metabolic syndrome with characteristic intra-abdominal obesity.更多还原