【摘要】 目的探讨他克莫司对大鼠肾缺血再灌注的保护作用。方法选用Wistar雄性大鼠60只,采用抽签法随机分为3组(每组20只):假手术组、对照组、实验组。到达设定的不同灌注时间点取血和肾脏标本,测定血清肌酐(creatinine,Cr)、尿素氮(blood urea nitrogen,BUN)、肿瘤坏死因子α(tumor necrosis factor,TNF-α);检测肾组织B细胞淋巴瘤(B cell lymphoma,Bcl-2)及病理情况。结果在缺血45 min后再灌注2、6和24 h时,实验组的Cr、BUN水平显著低于对照组(P<0.05),而Bcl-2免疫反应强度显著高于对照组(P<0.05)。在再灌注的各时间点,实验组的血清TNF-α含量水平均显著低于对照组(P<0.05)。HE染色光镜下对照组的肾小管及肾小管上皮细胞病变程度重;实验组的肾小管及肾小管上皮细胞病变程度明显减轻;假手术组肾小管及肾小管上皮细胞未见明显改变。结论他克莫司对大鼠肾缺血再灌注的保护作用可能是通过降低血清TNF-α表达水平和增加肾脏Bcl-2蛋白的表达,从而改善大鼠肾缺血再灌注损伤。更多还原

【Abstract】 Objective To study the protective effects of tacrolimus on the rat renal ischemia-reperfusion injury.Methods Sixty male healthy Wistar rats were assigned randomly into three groups: sham operation group,control group,and experimental group.In each group there were 20 rats.Blood and kidney specimens were taken in different reperfusion time,and serum creatinine(Cr),urea nitrogen(BUN),tumor necrosis factor(TNF-α) were determined;kidney tissues B cell lymphoma protein(Bcl-2) and pathological changes were detected.Results After 45 minutes of ischemia,reperfusion was performed at 2 h,6 h and 24 h in the experimental group serum creatinine(Scr),blood urea nitrogen(BUN) levels were significantly lower than those of the control group(P<0.05),however,Bcl-2 immune response intensity was significantly higher than those of the control group(P<0.05).As to reperfusion at the various time points,in the experimental group of TNF-α content was significantly lower than that of the control group(P<0.05).Under light microscope,the control group showed more severe pathological changes in renal tubules and tubular epithelial cells;the experimental group showed significantly less severe renal disease and renal epithelial cells pathological changes than the control group.Sham operation group did not show any significant tubular and tubular epithelial cell changes.Conclusion Tacrolimus on showed significant protective effects against renal ischemia-reperfusion protective effect that may reduce serum levels of TNF-α expression and increase the Bcl-2 protein expression to improve renal ischemia-reperfusion injury.更多还原