【摘要】 目的探究去甲肾上腺素对大鼠结肠黏液分泌的影响以及肾上腺能β受体在大鼠结肠黏膜的分布。方法采用酶联免疫吸附测定法检测去甲肾上腺素(norepinephrine,NE)对结肠黏液分泌的影响;用免疫荧光组织化学方法观察黏蛋白2(mucin2,MUC2)及β肾上腺能受体在结肠黏膜的分布;实时定量PCR方法检测β肾上腺能受体在大肠结肠黏膜的表达;同时采用阿利新蓝/过碘酸雪夫染色方法检测黏液细胞在结肠黏膜的分布;使用激光共聚焦显微镜观察β2肾上腺能受体与MUC2在结肠黏液细胞的共存。结果去甲肾上腺素可刺激大鼠远端结肠黏膜的黏液释放增加,比对照组增加约247%。免疫荧光组织化学结果显示β1和β3肾上腺能受体在结肠黏膜有弱表达,β2肾上腺能受体为高表达。β1和β2肾上腺能受体mRNA表达相对量与免疫荧光组织化学一致。黏蛋白MUC2主要表达在结肠隐窝的黏液细胞内,且与β2肾上腺能受体有共存。结论β2肾上腺能受体与MUC2在结肠黏液细胞共存;去甲肾上腺素可促进结肠黏液分泌。更多还原

【Abstract】 Objective To investigate the effect of noradrenalin on the colonic mucus secretion and the distribution of β adrenoceptors in the colonic mucous cells.Methods Enzyme-linked immunosorbent assay(ELISA) was used to investigate the effect of norepinephrine(NE) on the colonic mucus secretion;immunofluorescence and laser scanning confocal microscope were used to observe the distribution of mucin2(MUC2) and β adrenoceptors in rat colonic mucosa.The mRNA expression of the β adrenoceptors in the rat colonic mucosa was analyzed by real-time PCR.Alcian blue/periodic acid schiffs(AB/PAS) staining was used to detect the distribution of mucous cells.Results NE stimulated the secretion of colonic MUC2 from(0.19±0.03) ng/mg to(0.47±0.09)ng/mg,increased by 247%.The mRNA expression of β1 adrenoceptor and β3 adrenoceptor was weak and β2 adrenoceptor was the highest among the adrenoceptors expression in the colonic mucosa.Immunofluorescence staining results indicated the co-expression of MUC2 and β2 in the colonic mucous cells.Conclusion NE is able to stimulate muc2 release from rat distal colonic mucosa.更多还原