【摘要】 目的探讨放射性碘131(131I)标记基因重组鞭毛蛋白(rFliC)在乳腺癌荷瘤小鼠体内的生物分布特征及意义。方法逆转录聚合酶链反应(RT-PCR)、蛋白质印迹法(Western blot)及免疫荧光检测人乳腺腺癌细胞系(MCF-7)中rFliC的受体TLR5的表达;四氯二苯基苷脲(Iodogen)法131I标记rFliC,检测其放射化学纯度、稳定性、细胞摄取状况;制备MCF-7荷瘤鼠,注射131I-rFliC后,观察其生物学分布及肿瘤靶向性。结果①MCF-7表达TLR5的mRNA及蛋白;随着rFliC浓度的增加,TLR5 mRNA及蛋白表达逐渐减弱;②131I-rFliC标记率达95.19%;放射化学纯度为96.46%;在血清中稳定性高,可被MCF-7稳定摄取;③131I-iFliC主要经肝肾代谢,肿瘤靶向性明显,24 h靶/肌肉(T/M)放射性比值为7.09,可获得清晰放射自显影像。结论131I-rFliC肿瘤靶向明显,有望作为一种新型分子探针监测乳腺癌的发生发展。更多还原

【Abstract】 Objective To investigate the biodistribution and significance of 131I-rFliC in breast cancer-bearing mice.Methods ① The expression of TLR5 was assayed by the immunofluorescence method,RT-PCR and Western blot.② rFliC was labeled by 131I and the radiochemical purity,stability,cell uptake and efflux of 131I-rFliC were identified.③ MCF-7 bearing mice were prepared and injected with 131I-rFliC.The biodistribution of 131I-rFliC and tumor targeting were analyzed.Results ① MCF-7 could express TLR5 at the mRNA and protein levels.The expression of TLR5 was down-regulated by rFliC with a dose-dependent manner.② The labeling efficiency of 131I-rFliC was 95.19% and the radiochemical purity was 96.46%.131I-rFliC was more stable in serum and could be absorbed by MCF-7 stably.③ 24 h after injection with 131I-rFliC,T/M ratio was 7.09,and tumor tissue appeared clearly with the autoradiography.Conclusion Tumor-targeting of 131I-rFliC is obvious,and it may become a new molecular probe for the diagnosis and therapy of breast cancer.更多还原