【摘要】 目的检测SPOP蛋白在人肾透明细胞癌细胞株、肾透明细胞癌组织及正常肾组织中的表达,探讨其表达与肾透明细胞癌的关系。方法采用免疫细胞化学EnVision二步法检测肾癌细胞株A498中SPOP的表达情况。取山东大学第二医院泌尿外科肾癌患者手术标本,采用HE染色明确肿瘤病理分级,分别采用免疫组织化学EnVision二步法、蛋白质印迹法定性及定量检测肾透明细胞癌及正常肾组织中SPOP的表达水平。结果 SPOP在人肾癌细胞株A498、肾透明细胞癌组织中均为阳性表达,正常肾组织中SPOP表达为阴性。结论 SPOP可能是一种肾透明细胞癌的分子标记物,对其监测将有可能协助肾透明细胞癌的诊断。更多还原

【Abstract】 Objective To investigate the expression of SPOP in the renal clear cell cancer cell line,renal clear cell carcinoma and normal renal tissues,and to explore the relationship between SPOP expression and renal clear cell carcinoma.Methods The expression of SPOP in renal clear cell cancer cell line A498 was detected by immunocytochemistry(DAKO EnVision system).Surgical biopsy specimens of renal clear cell carcinoma and the surrounding normal renal tissues were taken from the patients in the Department of Urology Surgery,the Second Hospital of Shandong University.Diagnosis of tumor pathological grades was carried out after HE staining.The expressions of SPOP in renal clear cell carcinoma and normal renal tissues were detected by immunohistochemistry(IHC,DAKO EnVision system) and Western blot.Results SPOP was positively stained in the renal clear cell cancer cell line A498 by immunocytochemistry.Expression of SPOP was detected in renal clear cell carcinoma by IHC and Western blot,while SPOP in the normal renal tissues was uniformly negative.Conclusion SPOP may be a new tumor marker of renal clear cell carcinoma.Surveillance of SPOP expression may be helpful to the diagnosis of renal clear cell carcinoma.更多还原