【摘要】 利用睡菜带芽的嫩茎段作为外植体,切成0.5 cm长的片段,通过MS培养基和激素配比实验,筛选出了睡菜茎段愈伤组织诱导的最佳培养基配方.实验结果表明:适宜的消毒时间75%酒精30 s,升汞9 min;适宜的初代培养基为MS+BA 1.0 mg.L-1+NAA 0.10 mg.L-1;适宜的继代培养基为MS+BA 0.5 mg.L-1+NAA 0.05 mg.L-1;在培养基中添加1.5 g/L的多菌灵粉剂可以有效抑制真菌生长,诱导出愈伤组织后应在30 d内及时进行继代培养.更多还原

【Abstract】 The study takes fresh stem with bud from Menyanthes trifoliata as explants,then cut it into fragments about 0.5cm long.Through a series of optimization expriment for cultural medium composition of phytohormones,the best culture medium formula for callus induction of this plant’s stem fragments was selected.The results indicated that the young stem segment with buds was the best explant;the suitable sterilization time with 75% alcohol was 30s and with 1 g·L-1 HgCl2 9 min.MS+BA 1.0 mg·L-1+NAA 0.10 mg·L-1 was the proper medium for primary culture,and MS+BA 0.5 mg·L-1+NAA 0.05mg·L-1 was the proper medium for subculture.Adding 1.5 g/L of carbendazim powder to the medium containing could inhibit the growth of fungi effectively.The callus should be timely subcultured in 30 days after callus has been inducted.更多还原