The segmentation of microvessel is one of critical image processing techniques to evaluate property and dysfunction of microvasculature.Up to the present,has no specific segmentation method been presented to deal with microvessels in muscular tissue images.Presented segmentation methods for vessels are not competent for this type of images.In this study,we proposed a combinatorial strategy — TOSC algorithm.first,segment the image preliminarily with single threshold was segmented;second,the connections betwe...