【摘要】 用抗β8整合素单克隆抗体,检测星形胶质细胞氧糖剥夺-复氧模型,小鼠死后不同时间肾和脑组织β8整合素表达、定位的变化,绘制光密度平均值-死亡时间曲线,初步推断小鼠个体死亡时间.结果显示缺氧环境中,星形胶质细胞、肾小管上皮细胞和脑胶质细胞β8整合素表达量升高.细胞形态的变化以脑胶质细胞最为明显,β8整合素定位由细胞膜到细胞浆,最后随细胞膜破裂释放到细胞外基质.Image-Pro Plus分析显示,缺氧脑胶质细胞β8整合素表达量在48 h达最高值,绘制的光密度平均值-死亡时间曲线能初步推断小鼠个体死亡的时间.更多还原

【Abstract】 Anti-β8 integrin monoclonal antibody was employed to detect the expression and location ofβ8 integrin on astrocytes in a oxygen-glucose deprivation(OGD)-reoxygenation model,as well as in kidney and brain in the different time points after postmortem in mice,then a density(mean)- postmortem interval curve was drawn to speculate the postmortem interval of mice.It was showed:the expression ofβ8 integrin on astrocytes,tubular epithelial cells and glial cells was increased in hypoxia.The location ofβ8 integrin transfered from cytomembrane to cytoplasm,and finallyβ8 integrin was released to extracellular matrix along with fracture of cytomembrane.Image-Pro Plus analysis indicated thatβ8 integrin expression of glial cells peaked at 48 h in hypoxia.The curve of density(mean)- postmortem interval was able to speculate the postmortem interval accurately.更多还原