【摘要】 为探究草鱼呼肠孤病毒(Grass carp reovirus,GCRV)能否引起细胞凋亡,采用GCRV分别感染草鱼肾细胞(Ctenopharyngodon idellus kidney cell,CIK)和胖头鲤细胞(Fathead minnow cell,FHM),在不同时间观察细胞病变效应。结果表明,GCRV能感染这2种细胞并引起细胞病变,且CIK细胞对GCRV更为敏感;GCRV感染可以导致CIK细胞的快速融合并裂解,而FHM细胞感染后则很少有细胞融合;利用DAPI、Annexin V-FITC染色和末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记[terminal deoxynucleotidyl transferase(TdT)dUTP nick end labeling,TUNEL]技术进一步证实GCRV能够诱导FHM细胞发生凋亡,但不诱导CIK细胞发生凋亡。本研究发现GCRV可以诱导FHM细胞发生凋亡,并推测GCRV在感染不同类型的细胞时,可以通过不同机制促使细胞死亡,从而为进一步研究GCRV的致病机制提供线索。更多还原

【Abstract】 Virus infection is associated with apoptosis.In order to study whether Grass carp reovirus(GCRV) can induce apoptosis,CIK and FHM cells were infected by GCRV in this study,and the cytopathic effects were abserved at different times post-infection.It was shown that both of CIK and FHM cells can be infected by GCRV,and CIK cells were more susceptible to GCRV.GCRV infection can cause CIK cells fusion and lysis rapidly.However there was little cell fusions that were observed in infected FHM cells.Apoptosis was detected by DAPI staining,annexin V-FITC staining and TdT-dUTP labeling in infected FHM cells,but not in infected CIK cells.We considered that GCRV-infected CIK cells may die through non-apoptosis mechanism.Our results demonstrated that GCRV can induce apoptosis in FHM cells and indicated that GCRV may induce different cell type’s death by different mechanisms,which can help to further understand and study the pathogenesis of GCRV.更多还原